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	Provedor de dados BJMBR (2) Autor Boni-Mitake,M. (2) Rogero,J.R. (2) Spencer,P.J. (2) Aird,S.D. (1) Costa,H. (1) Nascimento,N. (1) Vassilieff,V.S. (1) Mais... Palavra-chave Chromatography (2) Bothrops jararacussu (1) Bothropstoxin-1 (1) Circular dichroism (1) Crotamine (1) Gamma radiation (1) Myotoxins (1) Purification (1) Toxicity (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Info:eu-repo/semantics/other (1) Ano 2001 (1) 1998 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última A single-step purification of bothropstoxin-1 BJMBR Spencer,P.J.; Aird,S.D.; Boni-Mitake,M.; Nascimento,N.; Rogero,J.R.. Bothrops venoms are complex mixtures of components with a wide range of biological activities. Among these substances, myotoxins have been investigated by several groups. Bothropstoxin-1 (Bthtx-1) is a phospholipase A2-like basic myotoxin from Bothrops jararacussu. The purification of this component involves two chromatographic steps. Although providing a pure material, the association of these two steps is time consuming and a single-step method using high performance chromatography media would be useful. In the present study, we describe a single-step purification method for Bthtx-1. Bothrops jararacussu venom was dissolved in 1 ml buffer. After centrifugation, the supernatant was injected into a Resource-S cation exchange column connected to an FPLC... Tipo: Info:eu-repo/semantics/other Palavras-chave: Myotoxins; Purification; Chromatography; Bothrops jararacussu; Bothropstoxin-1. Ano: 1998 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1998000900004 Effects of 60Co gamma radiation on crotamine BJMBR Boni-Mitake,M.; Costa,H.; Spencer,P.J.; Vassilieff,V.S.; Rogero,J.R.. Ionizing radiation can change the molecular structure and affect the biological properties of biomolecules. This has been employed to attenuate animal toxins. Crotamine is a strongly basic polypeptide (pI 10.3) from Crotalus durissus terrificus venom composed of 42 amino acid residues. It induces skeletal muscle spasms leading to a spastic paralysis of hind limbs in mice. The objective of the present study was to carry out a biochemical study and a toxic activity assay on native and irradiated crotamine. Crotamine was purified from C.d. terrificus venom by Sephadex G-100 gel filtration followed by ion-exchange chromatography, and irradiated at 2 mg/ml in 0.15 M NaCl with 2.0 kGy gamma radiation emitted by a 60Co source. The native and irradiated toxins... Tipo: Info:eu-repo/semantics/article Palavras-chave: Crotamine; Gamma radiation; Chromatography; Toxicity; Circular dichroism. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001001200004 Registros recuperados: 2 Primeira ... 1 ... Última

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